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Archive > Volume 21 Issue 3 > 2014,21(3):293-297. DOI:10.3872/j.issn.1007-385X.2014.03.010 Prev Next
Basic Research
Inhibitory effect and mechanism of curcumin on nasopharyngeal carcinoma C666-1 cells proliferation and apoptosis
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Abstract:
To examine the effect of curcumin on mTOR signaling, proliferation and apoptosis in nasopharyngeal carcinoma C666-1 cells in vitro. Methods: C666-1 cells were stimulated by curcumin at increasing concentrations (0, 10, 25, 50, and 100 μmol/L) for 24 h or at a concentration of 50 μmol/L for 0, 6, 12 h and 24 h. Cell proliferation was assessed with the Dojindo’s Cell Counting Kit-8 (CCK-8), cell apoptosis by a TUNEL-based assay and levels of AMPK, S6K, S6 proteins by Western blotting. Results: Curcumin inhibited C666-1 cell proliferation and induced C666-1 cell apoptosis in dose-and time-dependent manners. In parallel with changes in cell proliferation and apoptosis, levels of phosphorylated AMPK were increasing but levels of phosphorylated S6K1 and S6 proteins were decreasing with the dose of curcumin used; against the baseline levels arbitrarily set as 1 in C666-1 cells in the non-treatment control group, the fold of changes in levels of phosphorylated AMPK, S6K1 and S6 proteins was 3.87±1.38, 0.19±0.16 and 0.39±024, respectively, in cells treated with 50 μmol/L for 24 h and was 4.34±1.34, 0.059±0.043 and 0.11±0.095, respectively, in cells treated with 100 μmol/L for 24 h. Conclusion: Curcumin may inhibit nasopharyngeal carcinoma cell proliferation and induce nasopharyngeal carcinoma cell apoptosis through an mTOR signaling pathway-dependent mechanism.
Keywords:
curcumin ; nasopharyngeal carcinoma ; mammalian target of rapamycin ; C666-1 cell ; proliferation ; apoptosis
Project Supported:
Project supported by the National Natural Science Foundation of China(No.81302357), the Guangdong National Nautral Foundation(No. 2013040016493), and the Foundation for Distinguished Young Talents in Higher Education of Guangdong Province (No.2013LYM0075)
