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Archive > Volume 21 Issue 3 > 2014,21(3):309-313. DOI:10.3872/j.issn.1007-385X.2014.03.013 Prev Next
Basic Research
Levels of sentrin specific-protease-1 mRNA and protein in hepatocellular carcinoma cells with different invasive and metastatic capacities
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Abstract:
To determine mRNA and protein levels of sentrin-specific protease 1 (SENP-1) in human non-tumor hepatic cells and various types of human hepatocellular carcinoma (HCC) cells with different invasive and metastatic capacities. Methods: Low invasive HCC cell lines MHCC97L, high invasive HCC cell lines MHCC97H and HCCLM3, non-invasive HCC cell lines SMMC-7721and HepG2 and immortalized normal liver cell line HL-7702 were utilized. Their levels of SENP-1 mRNA and protein were determined by RT-PCR andquantitative real-time PCR and Western blotting respectively while the intracellular location of SENP-1 assessed by immunofluorescence staining. Results: RT-PCR and real-time PCR analyses showed that SENP-1 mRNA abundance was significantly different between the cell lines evaluated (F=5.658；P=0.042) and increased with the incisive capacity of the cells. protein (F=88.909, P=0.000) in HCC cell lines were increased significantly. Western blotting analysis showed that SENP-1 protein levels were significantly higher in all five HCC cell lines tested as compared the immortalized normal liver cell line (F=88.9, P=0.0001). Immumofluorescence staining revealed that SENP1 protein was localized predominantly in cytolymph and in a small quantity in the nucleus. Conclusion: The higher the invasive and metastatic capacities of hepatocellular carcinoma cells, the higher the SENP-1 mRNA and protein levels. This observation suggests that SENP-1 may promote hepatocellular carcinoma cell invasion and metastasis．
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Project Supported:
Project supported by the Science and Technology Project of Baiyun District of Guangzhou City（No.2011-KZ-56）
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