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Effect of Cecropin-XJ and it’s combination with chemotherapeutic agents on the proliferation and apoptosis of human esophageal cancer Eca109 cells
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Abstract:
Objective: To evaluate the effect of cecropin-XJ in itself or in combination with one of the commonly used chemotherapeutic agents on the proliferation and apoptosis of human esophageal carcinoma Eca109 cells in vitro. Methods：Eca109 cells were treated with cecropin-XJ, either in itself or in combination with adriamycin (ADM), cis-diamminedichloride platinum II (DDP) or cyclophosphamide (CTX). Twenty-four hours after treatment, cell viability was assessed by MTT assays, and apoptosis, reactive oxygen species (ROS) levels and mitochondrial membrane potential were assessed by flow cytometry. Results：Cecropin-XJ alone significantly inhibited Eca109 cell proliferation at the doses used (range from 1 to 50 μmol/L) (P<0.05). Compared with the cecropin-XJ mono-treatment, the combined treatment of cecropin-XJ with ADM, DDP or CTX were significantly more effective to induce Eca109 cell proliferation inhibition, apoptosis and reactive oxygen species production, and to decrease Eca109 cell mitochondrial membrane potential (P<0.05). In contrast, the combination of cecropin-XJ with CBP showed an antagonistic effect. Conclusion：Cecropin-XJ combined with ADM, DDP, and CTX respectively may inhibit proliferation and induce apoptosis of human esophageal carcinoma cells more effectively than cecropin-XJ in itself. The synergistic effect may be associated with changes in the intracellular ROS production and mitochondrial membrane potential.
Keywords:
cecropin-XJ ; esophageal cancer ; Eca109 cell ; doxorubicin ; carboplatin ; cisplatin ; cyclophosphamide ; proliferation ; apoptosis
Project Supported:
Project supported by the High-Technology Research and Development Program of Xinjiang（No. 201110101）
