Mobile website
Basic Research
Survivin- and caspase-dependent apoptosis of esophageal cancer Ec9706 cells after norcantharidin exposure
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
Objective: To study the effect of norcantharidin on apoptosis of esophageal cancer Ec9706 cells and the putative mechanism(s) underlying the effect. Methods: Ec9706 cells were treated with norcantharidin at 0, 5, 10, 20 or 40 μg/ml. Cell viability was determined by MTT assays and apoptosis, caspase-3 and survivin protein levels in the treated Ec9706 cells were assessed by flow cytometry, respectively, at 12, 24 and 48 h after treatment. Results: Norcantharidin inhibited the growth of Ec9706 cells in a dose- and time-dependent manner; the growth inhibition rate was (80.00±215)% at 40 μg/ml for 48 h. Norcantharidin induced apoptosis, significantly increased caspase-3 protein level (P<0.05) and significantly decreased survivin protein level (P<0.05) in Ec9706 cells in a dose- and time-dependent manner; the apoptosis rate was 38.57±1.76%at 20 μg/ml for 48h. Conclusion: Norcantharidin may induce apoptosis of esophageal cancer Ec9706 cells through downregulation of survivin expression and upregulation of caspase-3 expression.
Keywords:
Project Supported:
Project supported by the Natrural Science Foundation of Hebei Province (No. H2012206107), and the Key Specific Discipline Foundation of Higher Institutions of Hebei Province (No. \[2005\]52)
Clc Number:
