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Expression and clinical significance of macrophage migration inhibitory factor and VEGF in human gastric carcinoma tissues
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Abstract:
Objective:To determine the expression of macrophage migration inhibitory factor (MIF) and vascular endothelial growth factor (VEGF) in gastric carcinoma in association with clinicopathological features of the carcinoma lesions. Methods: Eighty-nine tumor tissue specimens were collected between 2008 and 2013 and four pairs of tumor tissue and corresponding adjacent tissue specimens were collected in 2013 from patients who were diagnosed with gastric carcinoma in Henan University-Affiliated Hospital. Contents of MIF and VEGF proteins in gastric carcinoma and adjacent tissue specimens were assessed by immunohistochemical staining and Western blotting. The correlation of MIF and VEGF levels with clinicopathological features of gastric carcinoma was analyzed by statistical analysis. Results: MIF and VEGF were detected in 88.8% and 50.6% of gastric carcinoma respectively, but were undetectable in normal tissue specimens. In the four tissue pairs, gastric carcinoma tissue had significantly higher levels of MIF (0.87±0.29 vs 0.23±0.14, P<0.05) and VEGF (0.89±0.23 vs 0.34±0.21, P<0.05) compared with the corresponding adjacent tissue. The expression of MIF and VEGF was not associated with age and sex but was positively correlated with the histological grade and TNM stage of gastric carcinoma (P<0.05). Conclusion: MIF and VEGF are highly expressed in gastric cancer and the expression level is correlated with the cancer staging.
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