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Effects of CD11b + NKT cells derived from poly-I:C-challenged mice on CD8 T cell proliferation and cytotoxicity
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Abstract:
Objective: To investigate the effect of expanded CD11b + NKT cells isolated from the injured murine liver following poly-I:C challenge on the proliferation and function of normal murine CD8 +T cells in vitro . Methods: Male C57BL/6 mice were treated with poly-I:C at 20 μg/g. CD11b + and CD11b - NKT cells were isolated from the liver 24 h after poly-I:C- treatment. CD8 +T cells were isolated from normal male OT-I mice and co-cultured with the isolated hepatic CD11b + and CD11b - NKT cells, respectively. The proliferation and cytotoxic ability of CD8 +T cells in the co-culture were both assessed by flow cytometry. The concentration of major immunoregulatory cytokines was determined by ELISA. Results: Poly-I:C treatment significantly increased the proportion of CD11b + NKT cells in the liver. After stimulation, CD11b + hepatic NKT cells produced less IFN-γ, IL-4 and IL-10 than CD11b - hepatic NKT cells. CD11b + hepatic NKT cells significantly inhibited both antigen-specific and nonspecific immune responses of CD8 +T cells, while CD11b - hepatic NKT cells showed no inhibitory effect. CD11b + hepatic NKT cells did not significantly alter the cytotoxic ability of activated CD8 +T cells. Conclusion: Poly-I:C-nduced liver injury is associated with the expansion of CD11b + hepatic NKT cells. While these CD11b + hepatic NKT cells have little effect on the cytotoxic activity of activated CD8 +T cells, they significantly inhibit CD8 +T cell proliferation.
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project supported by the National Laboratory Special Fund (No. 2060204)
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