Mobile website
Basic Research
Biological effects of miR-155 on human lung cancer 95D cell
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
Objective: To evaluate the effect of microRNA-155 (miR-155) on human lung cancer cell behaviors in vitro . Methods: A plasmid vector (p-miR-155) carrying the pri-miR-155 sequence amplified from genomic RNA of human lung cancer 95D cells by PCR was constructed. Lung cancer 95D cells were transiently transfected with p-miR-155. p-miR-155 mRNA abundance in 95D cells was assessed by real-time PCR before and after transfection. Cell proliferation and migration in control, mock-transfected and transfected 95D cells were assessed by CCK-8 assay wound assay respectively. Results: The abundance of miR-155 mRNA was increased significantly in 95D cells transfected with p-miR-155 than in mock-transfected and control cells (2.045±0.62 vs 0.76±0.62, 1±0.45; P <0.01). The proliferation was markedly inhibited in p-miR-155 transfectants as compared in mock-transfected and control cells (\[4670±689\]% vs \[3.70±1.40\]%, \[1.11±0.75\]%; P <0.01). The number of colonies formed was significantly decreased (\[12±3\] vs \[34±3\], \[35±3\]; P <0.01) and so was migration capability (\[110±5\] vs \[295±5\], \[325±5\]; P ＜0.01) in p-miR-155-transfected cells as compared with mock-transfected and control cells. Conclusion: A eukaryotic expression vector carrying human pri-miR-155 sequence is capable of effectively inhibiting lung cancer cell proliferation and migration, thus having a significant clinical implication.
Keywords:
Project Supported:
Projects supported by the National Natural Science Foundation of China（No. 81260398, No.31370918），and the Foundation for New Century Excellent Talents by the State Education Commission（No. NCET-12-0661）
Clc Number:
