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Effects of C-phycocyanin combined with all-trans retinoic acid on cervical cancer cells proliferation and apoptosis
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Abstract:
Objective: To investigate the effects of all-transretinoic acid (ATRA) and C-phycocyanin (C-PC), either each alone or two in combination on cervical cancer cell growth and apoptosis in vitro . Methods: Immortalized human cervical cancer HeLa cells were treated with ATRA and C-CP, either each at various concentrations or two in various dose combinations. After treatment for 48 h, cell viability was assessed by MTT assay, apoptosis by TUNEL assay, and changes in Caspase-3 and Bcl-2 protein contents by Western blotting and immunohistochemistry staining, respectively. Results: C-PC and ATRA each alone significantly inhibited the growth of HeLa cells; and IC 50 was 0.158±0.036 mmol/L for C-CP and 192.75±5.79 μg/L for ATRA ( P <0.05). When ATRA was combined with C-PC, the IC 50 was significantly lower than that when ATRA was used alone. Compared with non-treatment control, C-PC and ATRA each alone induced significant HeLa cell apoptosis ( P <0.05), and in the apoptotic effect was more pronounced when C-PC and ATRA were used together ( P <0.01). ATRA and C-PC each alone significantly decreased Bcl-2 protein content ( P <0.01) but significantly increased Caspase-3 protein content ( P <0.01) in Hela cells, and both changes became more significant when ATRT and C-CP were used together ( P <0.01). Conclusion: C-PC combined with ATRA may induce cervical cancer cell apoptosis by down-regulation of Bcl-2 gene and up-regulation of Caspase-3 gene more effectively than each alone.
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Project Supported:
Project supported by the National Natural Science Foundation of China (No.81471546, No. 81001346)，the Medical Health Science and Technology Development Plan of Shandong Province (No. 2011HZ023)，and the Science and Technology Development Plan of Qingdao City Public Realm(No. 2012-1-3-5-(4)-nsh)
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