Mobile website
Basic Research
Effect of RNAi-mediated silencing of nucleostemin gene on proliferation and apoptosis of human non-small cell lung cancer A549 cells
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
Objective: To determine the effect of silencing nucleostemin ( NS ) gene expression on proliferation and apoptosis of non-small cell lung cancer (NSCLC) A549 cells in vitro . Methods: The recombinant pcDNA4/C-NS-silencer targeting the NS gene was constructed. Human NSCLS A549 cells were transfected with pcDNA4/C-NS-silencer or the control vectorpcDNA4/C. NS mRNA abundance was assessed by real-time RT-PCR, cell proliferation by CCK-8 assay, and cell cycle progress and apoptosis by flow cytometric assay with Hoechst33258 staining in untransfected and transfected A549 cells. Results: Compared with control vector-transfected and untransfected A549 cells, A549 cells transfected with pcDNA4/C-NS-silencer had significantly lower NS mRNA abundance (0.166±0.024 vs 0.497±0.022, 0.505±0032, P <0.01) and a significantly lower proliferation activity (0.518±0.107 vs 0.855±0.102, 0.832±0.158, P <005). NS gene silencing resulted significant increases in the percentage of cells in G1/G0 phase, in chromatin condensation and fragmentation, and in apoptosis (\[34.80±6.77\]% vs \[9.70±1.50\]%, \[8.16±2.01\]% P <0.01), as compared with control vector-transfected and untransfected A549 cells. Conclusion: RNAi-mediated silencing of the NS gene results in human non-small cell lung cancer cell proliferation, G-1 arrest and apoptosis, thereby possessing a therapeutic potential.
Keywords:
Project Supported:
Project supported by the National Natural Science Foundation of China (No. 81372293, No.81241088), the Science Research Project of the Bureau of Education of Heilongjiang Province (No. 12531736), and the Science Research Project of the Bureau of Health of Heilongjiang Province (No. 2009-456)
Clc Number:
