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The relationship of microsatellite instability state with loss of mismatch repair proteins and clinical pathological characteristics in sporadic colorectal cancers
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Abstract:
Objective : To investigate the relationship of microsatellite instability (MSI) condition with the deletion of mismatch repair (MMR) proteins (MLH1, MSH2, MSH6, PMS2) and the clinical pathological characteristics in sporadic colorectal cancers. Methods: MSI of DAN samples from sporadic colorectal cancers was examined with multiplex fluorescent PCR. MMR proteins in sporadic colorectal cancer tissues were detected with SP immunohistochemistry (IHC) method. Results: In 75 cases of sporadic colorectal cancers, 21 cases (28%) had MSI, including MSI-H (19 cases) and MSI-L (2). MMR protein deletion was found in 16 cases (21.3%), of which 15 cases (93.75%) were MSI-H, and the other case (6.2%) was MSS. The rate of MMR protein deletion in MSI group (15/21，71.43%) was significantly higher than that of MSS group (1/54，1.9%, P<0.01). Notably, 4 cases with MSI-H (6.78%) and 2 cases (339%) with MSI-L did not have any NMR protein deletion. MSI in these patients was associated with age, mucus content of the adenocarcinoma, and tumor metastasis (P<0.01). MSI-H tends to occur in >50 years old individuals who have mucinous adenocarcinoma harboring MMR protein deletion and often have no distant metastasis of the cancer. Conclusion: The incidence of MSI is higher than that of MMR protein deletion in sporadic colorectal cancer. These with MSI-H have lower risk of metastasis and better prognosis. Therefore, detecting MSI is valuable for improving the prevention, diagnosis, and treatment of sporadic colorectal cancer, and for reducing its incidence and mortality.
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Project Supported:
Project supported by the Key Discipline Construction Fund of Educational Committee of Guangdong Province（No. GX9404），the Scientific Research Project of Administration of Traditional Chinese Medicine of Guangdong Province（No.20122081），and the Key Science and Technology Research Project of Zhanjiang City（No.2009C3101017）
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