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Expression and clinical significance of c-Myc and Bin1 in esophageal squamous cell cancer
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Abstract:
Objective : To investigate the expression of c-Myc and Bin1 in association with clinical characteristic in patients with esophageal squamous cell cancer (ESCC). Methods: Carcinoma and para-carcinoma tissue specimens were collected from 54 patients with esophageal squamous cell cancer who underwent esophagectomy in Fourth Hospital of Hebei Medical University between April, 2013 and March, 2014. Bin1 and c-Myc mRNA and protein levels were determined by RT-PCR and immunohistochemistry respectively. The associations between c-Myc and Bin1 protein levels and clinical features were analyzed. Results: Compared with para-carcinoma tissue specimens, carcinoma tissue specimens had significantly higher c-Myc mRNA abundance (0.34±0.29 vs 0.17±0.16, P <0.001) and a significantly higher percentage of c-Myc protein-positive tests (55.56% vs 33.33%, P =0.033). In contrast, Bin1 mRNA abundance was significantly decreased ( P <0.001) in carcinoma tissue specimens (0.25±0.19) as compared with para-carcinoma tissue specimens (0.33±0.20) and the percentage of Bin1 protein-positive tests was significantly lower in carcinoma specimens than in para-carcinoma specimens 57.41% vs (84.48%, P =0.007). The abundance of c-Myc mRNA was negatively correlated with Bin1 mRNA abundance ( r =-0.790, P <0.001) and c-Myc protein level was negatively correlated with Bin1 protein level ( P =0.019). Both c-Myc and Bin1 proteins were associated with TNM stage, tumor invasion depth, tumor differentiation and lymph node metastasis. Conclusion: In ESCC tissue, the expression of c-Myc is up-regualted while the expression of Bin1 is down-regulated. Bin1 is negatively correlated with c-Myc at both mRNA and protein levels. The aberrations in both c-Myc and Bin1 is closely associated with tumor invasion and lymph node metastasis of ESCC.
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Project Supported:
Projects supported by the National Natural Science Foundation of China （No.81201607）, the Foundation for Distinguished Young Scientists of Hebei Province（No. H2014206320）, and the Natural Science Foundation of Hebei Province（No.H2012206135）
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