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Efficacy and safety of dendritic cell-induced cytotoxic T lymphocytes in the treatment of patients with HPV-positive cervical cancer
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Abstract:
Objective : To evaluate the efficacy and safety of dendritic cell (DC)-induced cytotoxic T lymphocytes (CTLs) in the treatment of patients with HPV-positive cervical cancer (CC). Method: Peripheral blood mononuclear cells (PBMCs) were collected from 8 HPV-infection CC patients who were admitted to our institute between November, 2010 and May, 2012. CTLs were generated by infecting the isolated PBMCs with adenoviral vector carrying HPV16/18 E6/E7 together with sequential stimulations with GM-CSF IL-4, TNF-α, IL-2 and IL-7 for once two weeks as an infusion. Patients were each treated with their own adenovirus-infected DC-CTLs through intravenous infusion twice a week for 3 weeks. Follow-up was performed for 6-27 months, during which patients’ response and adverse events were assessed. Result: Out of the 6 patients with partial remission/stable disease prior to treatment, 5 became HPV-negative. Adenovirus-infected DC-CTLs resulted in complete remission in 1 patient with partial remission prior to treatment but showed little effect in the 2 patients with a progressive disease prior to treatment. No significant adverse events were observed in any of the patients. Conclusion: Autologous transfusion of CTLs induced by DCs overexpressing HPV16/18 E6/E7 may offer a novel efficient and safe treatment for HPV-positive CC patients under a partial remission/stable disease state.
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Project supported by the Major Science and Technology Foundation of “Twelve Five-year Plan” of China（No. 2009zx09503）
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