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Establishment of a mouse model of hematogenous dissemination of circulating hepatocellular carcinoma cells transplanted via rapid and vast tail vein injection
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Abstract:
Objective : To establish a mouse model of intrahepatic recurrence and metastasis of circulating hepatocellular carcinoma cells transplanted via rapid and vast tail vein injection. Methods: C57BL/6J mice were randomly divided into a control group ( n =20) and an experimental group ( n =20). Animals in the control group were given 2×10 6 human hepatoma Hepa1-6 cells/0.2 ml through conventional speed (30 s) tail vein injection and those in the experimental group were given 2×10 6 Hepa1-6 cells/0.2 ml through rapid (5 s) and vast tail vein injection. Four weeks later, animals were sacrificed, liver, lung, kidney and spleen were collected for H-E staining, and tumor formation was evaluated grossly and microscopically. Results: In the control group, tumor nodules were seen in 19 (95%) mice and the metastatic lesions occurred only in the lung but not in liver, spleen and kidneys. In the experimental group, tumor nodules were present in the lungs in 18 (94.7%) mice and in the liver in 19 (100%) mice while no metastatic tumors were found in the spleen and kidneys. Conclusion: Rapid and vast tail vein injection of hepatoma carcinoma cells in to nude mice may mimic the process of hematogenous dissemination of the residual circulating hepatocellular carcinoma cells after curative resection, thus offering a potentially useful, simple, efficient and safe animal model to study the metastasis of the circulating residential hepatoma carcinoma cells after resection.
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Project Supported:
Project supported by Grants from China National Key Projects for Infectious Disease (No.2012ZX10002012-10), and the National Natural Science Foundation of China (No.81272669, No. 81301830)
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