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Basic Research
Effect of Tanreqing on chemotherapy-associated immune suppression in mice with experimentally induced Lewis lung carcinoma
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Abstract:
Objective:To investigate the effect of an injectable Chinese herbal medicine Tanreqing on chemotherapy- induced immunosuppression in a mouse model of lung carcinoma. Methods: A total of 40 C57 BL/6 mice were randomized to five groups: 1) normal control, 2) experimentally induced lung carcinoma, 3) Tanreqing treatment, 4) chemotherapy, and 5) chemotherapy combined with Tanreqing treatment. The designated treatments commenced 10 days after lung carcinoma induction and lasted for 5 days. At the end of the treatments, animals were sacrificed after periphery blood sampling, spleen and thymus were collected, and macrophages were isolated from peritoneal fluid. Thymus index and lymphocytes in peripheral blood were quantified. Apoptosis rate of thymus cells and proportion of CD3+, CD3-NK1.1+ and CD3+NK1.1+ cells in peripheral blood lymphocytes were determined by flow cytometry. The viability of T and B lymphocytes were assessed by MTT assay. Bcl-2 and Fas mRNA levels in thymus were measured by RT-PCR. The cytotoxicity of peritoneal macrophages and their secretion of IL-12 were determined with LDH and ELISA kits. Serum levels of IgG and C3 were measured by immune turbidimetry. Results: As compared with the chemotherapy group, the chemotherapy and Tanreqing combination group had significantly higher thymus index, lymphocyte count and T and B lymphocyte viabilities in the spleen ( P <0.01), significantly higher percentages of CD3+, CD3-NK1.1+ and CD3+NK1.1+ cells in peripheral-blood lymphocytes ( P <0.01), and significantly higher Bcl-2 mRNA abundance ( P <0.01) but significantly lower Fas mRNA abundance ( P <0.01).Peritoneal macrophages isolated from animals in the combination treatment group produced significantly higher levels of IL-2 and displayed significantly higher cytotoxicity than those isolated from animals in the chemotherapy group ( P <0.01). Moreover, serum levels of IgG and C3 were significantly higher in the combination group than in the chemotherapy group ( P <0.01). Conclusion: The injectable Chinese herbal medicine Tanreqing appears to have an immune protective and anti-tumor immunity-enhancing activities in lung carcinoma patients undergoing chemotherapy.
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Project Supported:
Project supported by the Foundation of Traditional Chinese Medicine Administration of Hebei Province (No. 2013018)
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