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Cyclin G2 in thyroid carcinoma: Expression and effect on proliferation and apoptosis
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Abstract:
Objective:To analyze the expression of cyclin G2 (CCNG2) in thyroid carcinoma tissue specimens and evaluate the effect of CCNG2 on the proliferation and apoptosis of thyroid carcinoma SW579 cells in vitro . Methods: Sixty-three patients diagnosed with thyroid carcinoma in the Department of Endocrinology of Tangshan Workers Hospital between 2003 and 2008 were recruited. Biopsy specimens were collected from primary tumors and the corresponding adjacent tissues. CCNG2 protein presence and content in these specimens were determined by immunohistochemistry and Western blotting. The relationships of CCNG2 expression with clinicopathologic variables and patient survival were assessed. To evaluate the effect of CCNG2 on thyroid carcinoma cell proliferation and apoptosis in vitro , SW579 cells were transfected with a lentiviral vector expressing CCNG2 and the transfectants were then subjected to reverse transcription-polymerase chain reaction (RT-PCR) determination of CCNG2 mRNA abundance, Western blotting analysis of CCNG2 protein content, MTT assay of cell viability and flow cytometric assessment of apoptosis, respectively. Results: CCNG2 protein was detected at significantly lower levels in thyroid cancer tissue than in the paraneoplastic tissues (0.343±0.033 vs 0.713±0.062, P <0.05). While the level of CCNG2 protein was not correlated with gender, age, and tumor size ( P >0.05), it was significantly correlated with lymph node metastasis, clinic stage, histological grade and 5-year overall survival ( P <0.05). Overexpression of CCNG2 significantly lowered SW579 cell viability and enhanced SW579 cell apoptosis (15.1±2.3)% as compared with the control (5.6±1.1 in vitro , P <0.05). Conclusion: CCNG2 protein content is significantly lower in thyroid carcinoma tissue than in peritumor tissue. Overexpression of CCNG2 in thyroid carcinoma cells in vitro results in a significant inhibition of proliferation and a significant enhancement of apoptosis. These observations suggest that CCNG2 may serve as a potential therapeutic target for thyroid carcinoma.
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