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Ginsenoside Rg3 inhibits gastric cancer cell proliferation through Ca 2+ /CaM kinase downregulation and NF-κ B inactivation
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Abstract:
Objective: To study whether and how ginsenoside Rg3 regulate apoptosis of gastric cancer BGC-823 cells. Methods: BGC-823 cells were treated with vehicle (control), Rg3 (50 μg/ml), Ad-CaM, and Rg3 (50 μg/ml) plus Ad-CaM, respectively. At 48 hours after treatment, cell viability was assessed by MTT assay, cell apoptosis by flow cytometry, cell invasive capacity by trans-well assay, CaM, Iκ B, CaMKⅡ and NF-κ B protein contents by Western blotting analysis. Results: Compared with vehicle control, Rg3 significantly promoted apoptosis and inhibited proliferation and invasion of BGC-823 cells (P<0.05), while Ad-CaM and Rg3+Ad-CaM significantly inhibited apoptosis and promoted proliferation and invasion of BGC-823 cells (P<0.05). At the protein level, the expression of NF-κ B and CaMKⅡ was significantly downregulated whereas the expression of IκBα was significantly in Rg3-treated BGC-823 cells (P<0.05), and the expression of NF-κ B and CaMKⅡ was significantly enhanced whereas the expression of IκBα was significantly inhibited in cells treated with Ad-CaM and Rg3 plus Ad-CaM respectively (P<0.05), as compared with control cells. Conclusion: Rg3 may inhibit proliferation and invasion and promote apoptosis of gastric cancer cells through downregulation of CaM kinase expression and inactivation of NF-κ B.
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Project supported by the Natural Science Foundation from Science and Technology Department of Liaoning Province (No. 2014022044),and the Principal Foundation of Liaoning Medical Univercity (No. XZJJ 20130230)
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