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Archive > Volume 22 Issue 4 > 2015,22(4):432-437. DOI:10.3872/j.issn.1007-385X.2015.04.004 Prev Next
Basic Research
STAT3 activation is involved in the interaction of glioma and human brain microvascular endothelial cells
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Abstract:
Objective:To explore the role ofSTAT3 signaling pathway in the interaction of glioma cells and human brain microvascular endothelial cells (HBMEC). Methods: The secretion of IL-6 from HBMEC co-cultured with VEGF，A172, or U251 cells by Elisa. Immunoblotting was performed to examine STAT3 and its phosphorylation status inglioma cells after exposed to IL-6(50 ng/ml), co-cultured with the endothelial cells, orHBMEC+AZD1480. CCK-8 and Transwell assays were used to measure the effect of IL-6 (50 ng/ml), HBMEC, and HBMEC+AZD1480 on the proliferation and invasion of glioma cells A172 and U251. Results: Glioma cells and VEGF promoted the secretion of the IL-6 bythe endothelial cells(P<0.01). The level of pSTAT3 in A172 and U251 was significantly increased after exposed to IL-6， HBMEC， or HBMEC+AZD1480 (P<0.01). Moreover, AZD1480 reduced STAT3 phosphorylationin A172 and U251 co-cultured with HBME(P<0.01). IL-6 (50ng/ml) and co-culturing with the endothelial cells enhanced the growth and invasion of the glioma cells(P<0.01), where as AZD1480 suppressed the growth and invasion of the A172 and U251 cells (P<001). Conclusion: STAT3 activation is involved in the interaction between glioma cells and the human brain microvascular endothelial cells.
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Project Supported:
Project supported by the National Natural Science Foundation（No. 81160312）
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