Mobile website
Archive > Volume 22 Issue 4 > 2015,22(4):454-458. DOI:10.3872/j.issn.1007-385X.2015.04.008 Prev Next
Basic Research
Effects of siRNA-mediated Semaphorin 4D silencing on proliferation and migration of human breast cancer MDA-MB-231 cells
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
Objective:To investigate the effects of siRNA-mediated Semaphorin 4D (Sema 4D) gene silence on the proliferation and migration of breast cancer MDA-MB-231 cells. Methods:Small inference RNA targeting different regions of Sema 4D gene were transfected into MDA-MB-231 cells by lipofectamine 2000. The most effective siRNA in knockdown Sema 4D was screened by using quantitative PCR and Western blotting. The changes of cell proliferation and migration ability after Sema 4D knockdown were examined by CCK-8 and transwell assays. Results: MDA-MB-231 cells transfected with Sema 4D siRNA had significantly decreased expression of Sema 4D, especially in these transfected with siRNA-C (P<005). Compared with the blank and scrambled siRNA transfection groups, siRNA-C transfection significantly inhibited the proliferation of MDA-MB-231 cells (P<0.05) and their migration ability （numbers of migration cell：\[19620±904\] and \[186.40±6.69\] vs \[105.60±12.07\]，P<0.05). Conclusion: Down-regulation of Sema 4D by siRNA significantly inhibits the proliferation and migration of MDA-MB-231 cells, suggesting Sema 4Dis a novel target for breast cancer therapy.
Keywords:
Project Supported:
Project supported by the National Natural Science Foundation of China (No. 81271979)
Clc Number:
