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Archive > Volume 22 Issue 4 > 2015,22(4):495-499. DOI:10.3872/j.issn.1007-385X.2015.04.015 Prev Next
Clinical Research
Expression and clinical significance of HIF-1α and GLUT-1 in human nasopharyngeal carcinoma
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Abstract:
Objective : To study the expression and clinical significance of HIF-1α and GLUT-1 in human nasopharyngeal carcinoma (NPC). Methods: The expression of HIF-1α and GLUT-1 were examined in tumor tissues collected from 79 patients with NPC enrolled into our hospital from September 2010 to October 2013 and in nasopharyngitis tissues from 45 patients by using immunohistochemical staining SP method. MVD was counted by immunostaining with anti-CD34 antibody. The expression of HIF-1α, GLUT-1, and VEFG were also determined in CNE1 cells cultured under hypoxia condition. Results: Immunohistochemical staining showed that HIF-1α was highly expressed in nuclei of NPC (positive rate 63.40%) but not in nasopharyngitis tissues. The level of HIF-1α expression was associated with tumor size，lymph metastasis, T stage and clinic stage (P<0.05). HIF-1α expression was also positively correlated with MVD, which was significant higher in NPC. GLUT-1 was weakly expressed in nasopharyngitis tissues (positive rate 6.67%) but strongly expressed in NPC tissues (positive rate 53.16%). The expression of GLUT-1 in NPC was associated with MVD, lymph metastasis and clinic stage (P<0.05). Additionally, hypoxia increased the levels of HIF-1a, GLUT-1 and VEGF in CNE1 cells. Conclusion: The expression of HIF-1α and GLUT-1 are elevated in NPC, which are associated with pathological features and increased MVD. The in vitro study supported the notion that HIF-1α promotes the growth of nasopharyngeal cancer blood vessels through activating VEGF production. These results also suggest that HIF-1a and GLUT-1 are potential targets for the diagnosis and treatment of NPC.
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Project Supported:
Project supported by the Transformation of Scientific Achievements Project of Nanyang City (No. 2013021-18W2), and the Science and Technology Research Project of Nanyang City (No. 2010014-18WG)
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