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Archive > Volume 22 Issue 5 > 2015,22(5):566-572. DOI:10.3872/j.issn.1007-385X.2015.05.003 Prev Next
Basic Research
Tumor antigen MAGE-A11 increases ER-mediated cell proliferation of breast cancer MCF-7 cells
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Abstract:
Objective:To investigate the effect of melanoma antigen gene-A11 (MAGE-A11) on estrogen receptor (ER)-mediated cell proliferation of breast cancer MCF-7 cells.Methods:RT-PCR and Western blotting confirmed ER-positive MCF-7 cells were transfected with pCMV-AC-MAGE-A11-GFP and pCMV-AC-GFP respectively. Wild-type and transfected MCF-7 cells were treated with 17β-estradiol (17β-E). After treatment, estrogen-responsive finger protein (Efp) was assessed by RT-PCR and Western blotting, protein interaction between MAGE-A11 and ER by immunoprecipitation, cell viability by MTT assay and colony-forming capacity by colony formation assay.Results: Treatment with 17β-E significantly increased Efp mRNA and protein proteins in both wild-type and transfected MCF-7 cells (P<0.05). Evident interactions between MAGE-A11 and ER were detected in both wild-type and transfected MCF-7 cells. Estrogen treatment also significantly increased cell viability and colony formation in wild-type and MAGE-A11-transfected MCF-7 cells (P<005). Conclusion: In ER-positive MCF-7 cells, MAGE-A11 is capable of enhancing the expression of the ER target gene Efp and cell proliferation through direct interaction with ER. This finding suggests that MAGE-A11 may offer a potential therapeutic target for resistant ER-positive breast cancer.
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Project Supported:
Project supported by the National Natural Science Foundation of China (No.81001178), and the Hebei Science and Technology Supporting Program（No. 14277732D）
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