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Archive > Volume 22 Issue 5 > 2015,22(5):578-583. DOI:10.3872/j.issn.1007-385X.2015.05.005 Prev Next
Basic Research
Effect of miR-145-inhibited c-Myc expression on nasopharyngeal carcinoma cell proliferation
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Abstract:
Objective:The aim of this study was to investigate the possible mechanisms underlying the effect of miR-145 on the proliferation in nasopharyngeal carcinoma (NPC) cells.Methods: Protein and mRNA levels of miR-145 and c-Myc mRNA and protein were determined in 3 NPC cell lines (CNE-1, CNE-2 and CNE-2Z) and one immortalised nasopharyngeal epithelial cell line (NP69) by Western blotting and Real-time PCR respectively. To evaluate the effect of miR-145 on c-Myc transcript and proliferation in NPCs, CNE-1 cells were transfected with microRNA mimics and small interfering RNA using LipofectamineTM 2000. Transfectants were subjected to proliferative activity assessment using Cell Counting Kit-8 assay and cell cycle arrest analysis by propyliodide organism (PI) staining.Results: The expression of miR-145 in was down regulated but c-Myc expression was up-regulated in all NPC cell lines studied as compared with NP69 cells. After Transfection of miR-145 mimics resulted in significant growth-suppression(P<0.01) and a significant increase in cell cycle arrest in G1 phase (P<0.05). Knock down of c-Myc significantly inhibited CNE-1 cell proliferation (P<001), and resulted in increased accumulation of CNE-1 cells in G1 phase (P<0.05). Furthermore, miR-145 inhibited c-Myc expression in CNE-1 cells by directly affecting the 3’ untranslated region (3' UTR) of the c-Myc gene. Conclusion: miR-145 may inhibit NPC cell proliferation, possibly through a direct effect on the c-Myc 3’ UTR region. This observation may have significant implications in the diagnose and treatment of NPC.
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Project Supported:
Project supported by the Natural Science Foundation of Jiangsu Higher Education Institutions（No. 14KJB310017），and the Innovation Project of Suzhou International Technology Transfer and Cooperation（No. SH201209）
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