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Archive > Volume 22 Issue 5 > 2015,22(5):607-611. DOI:10.3872/j.issn.1007-385X.2015.05.010 Prev Next
Basic Research
Synergistic inhibition effects of cyclosporin A and cisplatin against lung cancer: a pilot study in nude mice
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Abstract:
Objective:The purpose of this study was to evaluate the effect of cyclosporine A (CsA) plus cisplatin (DDP) on lung cancer growth in vivo. Methods: Lewis lung carcinoma 3LL cells (100 μl of 5×106/ml) were injected subcutaneously into female nude (C57BL/6) mice. Two days after xenograft injection, animals were randomized to four treatment groups (n=10): Control, cisplatin (DDP), cyclosporine A (CsA), and DDP + CsA. DDP and CsA were intraperitoneally administered initially at 2 mg/kg, q3d for 3 times and at 5 mg/kg, q2d for 3 times, respectively, and once a week afterwards. Tumor size was examined every three days. The weight of tumor mass was calculated. Animals were sacrificed 35 days after xenograft implantation. Tumors were collected and weighed. Tumor tissue specimens were subjected to H-E staining immunohistochemical assessment of Ki-67 expression. Results:Compared with the control, all drug treatments significantly reduced tumor size (P<0.01), tumor weight (P<0.05), cell density and mitotic count (P<005)and Ki-67 expression (P<0.05), but DDP together with CsA was significantly more effective than DDP and CsA each alone (P<0.05). Conclusion:The combined use of CsA and DDP may increase the sensitivity of lung carcinoma cells to DDP and thus have a synergistic effect against lung cancer.
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