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Archive > Volume 22 Issue 5 > 2015,22(5):612-618. DOI:10.3872/j.issn.1007-385X.2015.05.011 Prev Next
Clinical Research
Distribution of LAP+CD4+ T cells in colorectal cancer microenvironment and its clinical significance
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Abstract:
Objective:This study aimed: (1) to determine the distribution of LAP+CD4+T cells in the tumor microenvironment of colorectal cancer (CRC); (2) to analyze the associations of LAP+CD4+T cells with CD4+CD25+Treg and clinicopathological variables; and (3) to evaluate the role for LAP+CD4+T cells in CRC development and progression. Methods: Clinicopathologic data of 50 CRC patients who were hospitalized and received surgical treatment in the Colorectal and Anal Department of Guangxi Medical University-Affiliated First Hospital of between January, 2014 and March, 2014 were collected. Preoperative peripheral blood, intraoperative tumor tissue and corresponding para-carcinoma tissue (over 10 cm from the tumor margins) specimens were collected for all patients. Peripheral blood samples were collected from 25 healthy volunteers for the purpose of control. Proportions of LAP+CD4+T cells and CD4+CD25+Treg in all samples were determined by flow cytometry. Associations of LAP+CD4+T cells with CD4+CD25+Treg and clinicopathological variables were analyzed. Results: The proportion of LAP+CD4+ T cells in the peripheral blood was significantly higher in CRC patients (9.44±3.18%) than in healthy controls (1.49±1.00%) (P=0.000), and was also significantly higher in tumor tissue (11.76±3.74%) than in the corresponding para-carcinoma tissue (3.87±1.64%) in CRC patients (P=0.000). LAP+CD4+T cells were positively correlated with CD4+CD25+Treg cells in tumor tissues (r=0.327, P=0.02) and also with TNM staging, distant metastasis and CEA levels (P<0.05). Conclusion: LAP+CD4+T cells are prone to aggregate in the CRC tissue, thus promoting CRC growth and metastasis.
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Project Supported:
Project supported by the National Natural Science Foundation of China(No. 81260316)，and the Innovation Project of Guangxi Graduate Education（No.YCBZ2014032）
Clc Number:
