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Archive > Volume 22 Issue 5 > 2015,22(5):619-624. DOI:10.3872/j.issn.1007-385X.2015.05.012 Prev Next
Clinical Research
Expression and clinical significance of IL-6 and VEGF in esophageal squamous cell cancer
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Abstract:
Objective:To study changes in serum levels of interleukin (IL)-6 and vascular endothelial growth factor (VEGF) in associations with tumor invasion and lymphatic metastasis in patients with esophageal squamous cell cancer (ESCC). Methods: Tumor and peri-tumor tissue specimens and peripheral blood samples were collected from 52 ESCC patients who underwent surgical resection in the Department of Chest Surgery, Hebei Medical University-Affiliated Fourth Hospital between January, 2014 and January, 2015. Blood samples were collected from 52 healthy subjects for the purpose of control. IL-6 levels in the blood samples were determined by enzyme-linked immunosorbent assay (ELISA). VEGF protein in the tumor and peri-tumor specimens was assessed by immunohistochemistry. IL-6 and VEGF mRNA abundance was assessed by semi-quantitative RT-PCR. Results: Serum levels of IL-6 were significantly higher in ESCC patients (116.71±25.98) pg/ml than in control subjects (78.43±9.36) pg/ml (P<0.05). In ESCC patients, serum levels of IL-6 were associated to patient TNM stage, tumor invasion depth, tumor differentiation and lymph node metastasis (P<0.05). VEGF protein was detected in 67.31% of tumor specimens and the intensity of the immunoreactive protein signal was associated to TNM stage, tumor invasion depth, tumor differentiation and lymph node metastasis. Tumor tissue VEGF was associated to serum level of IL-6 (P<0.05). IL-6 and VEGF mRNA levels were positively correlated in the tumor tissue (r=7.113, P<0.05). Conclusion: IL-6 is positively correlated with VEGF in ESCC patients. This finding suggests that IL-6 and VEGF may play key roles ESCC invasion and lymph node metastasis.
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Project Supported:
Projects supported by the National Natural Science Foundation of China (No.81201607), the Foundation for Distinguished Young Scientists of Hebei Province (No. H2014206320)，and the Foundation for High-level Talents of Hebei Province (No. A201401040)
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