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Archive > Volume 22 Issue 5 > 2015,22(5):625-629. DOI:10.3872/j.issn.1007-385X.2015.05.013 Prev Next
Clinical Research
Expression and clinical significance of heat shock protein 47 in gliomas
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Abstract:
Objective:To investigate the expression profile of heat shock protein-47 (HSP47) in glioma and evaluate the diagnostic and prognostic significance of HSP47 in clinical settings.Methods: Biopsy specimens were collected from 92 patients diagnosed with glioma and 10 patients diagnosed with gliosis between January, 2008 and December, 2009 at General Hospital of Shenyang Military Command. Additional 15 frozen glioma specimens collected between May, 2014 and June, 2014 were obtained. Immunoreactive HSP47 protein signals in these specimens were assessed by immunohistochemical staining and Western blotting. The prognostic significance of HSP47 was assessed by Kaplan-Meier survival analysis. Results:HSP47 was positive in 52.17% of glioma specimens but was undetectable in non-glioma specimens (χ2=9.855, P=0.002). In HSP47-positive Ⅲ、Ⅳ-grade glioma specimens, the immunoreative HSP47 protein signal was mainly located the cytoplasm of vascular endothelial cells. HSP47 expression was significantly associated with the pathological grade of the lesion (P<0.05) but not with gender (P=0.423) and age (P=0.820). The medium survival time was significantly longer in patients with low levels of HSP47 (43 months, 95 % CI, 22.4～63.6) than in patients with high levels of HSP47 (17 months, 95 % CI, 14.5～19.5) (P<0.001). Conclusion:HSP47 is overexpressed in glioma and its expression level is positively correlated with the pathological grade of the lesion. Our obervations suggest taht HSP47 may serve as a prognostic factor and a therapeutic target.
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Project supported by the Nature Science Foundation of Liaoning Province (No. 201202240)
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