Mobile website
Archive > Volume 22 Issue 6 > 2015,22(6):684-689. DOI:10.3872/j.issn.1007-385X.2015.06.002 Prev Next
Monographic Report
The apoptotic effect of sunitinib on human hepatocellular carcinoma cell line HepG2 and its mechanism
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
Objective:To investigate the apoptotic effect of sunitinib on human hepatocellular carcinoma cell HepG2 and explore the underlying molecular mechanism. Methods: The HepG2 cells were cultivated by routine method. The effect of sunitinib on the growth of HepG2 cells was assessed by MTT assay. Molecular targets in the hepatocellular carcinoma HepG2 cells were examined by immunoblotting. Apoptotic cell death was detected using Annexin-V/PI double labeled flow cytometry and TUNEL assay. The expressions of mRNA were quantitated by RT-qPCR. Results: The IC50 of sunitinib for inhibiting HepG2 cell growth was （3.22±0.50）μmol/L. After exposed to sunitinib, the expression of VEGFR1, VEGFR2, PDGFRα, Kit, FLT3 were decreased in HepG2 cells. Apoptosis rates of HepG2 cells were (5.90±0.45)% vs (15.18±1.28)% in the absence or presence of sunitinib respectively, and corresponding apoptosis index (AI) were (4.17±0.64) vs (23.54±4.73). After treated with sunitinib, the expressions of pro-apoptotic genes Bax, NOXA, PUMA and P53 were increased in the cells, whereas that of apoptosis-inhibiting genes Bcl-2 and X-IAP were significantly decreased.Conclusion: Increased expression of pro-apoptotic genes and decreased apoptosis-inhibiting genes are likely responsible for sunitinib-induced apoptosis in human hepatocellular carcinoma HepG2 cells.
Keywords:
Project Supported:
Project supported by the National Natural Science Foundation for Young Scientists of China (No. 81302372,81300431), and the Excellent Middle and Yong Aged Experts Project of Zhujiang Hospital Southern Medical University（No. 201207008)
Clc Number:
