Mobile website
Archive > Volume 22 Issue 6 > 2015,22(6):690-695. DOI:10.3872/j.issn.1007-385X.2015.06.003 Prev Next
Monographic Report
Sunitinib increases the expressions of NKG2DLs on human hepatocellular carcinoma HepG2 cells and enhances the cytotoxic action of NK cells
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
Objective:To investigate the effect of sunitinib on natural killer group 2 member D ligands (NKG2DLs) expression and NK-mediated cytotoxicity in human hepatocellular carcinoma cells. Methods: HepG2 cells were cultivated by routine method. Human NK cells were isolated by magnetic activated cell sorting (MACS). Flow cytometry was used to evaluate the purity of the isolated NK cells and the expression levels of NKG2DLs on HepG2 cells. The cytotoxic effect of NK cells against HepG2 was assessed with LDH releasing assay. The expressions of NKG2DL mRNAs in HepG2 cells was quantitated by RT-qPCR. Results: More than 78% of the isolated cells were CD3-CD16+CD56+, indicative of NK cells. After sunitinib treatment, the expressions of multiple NKG2DLs on HepG2 cells were increased, especially that of MICA, MICB and ULBP2. At the E∶T ratio of 10∶1 and 20∶1, the cytotoxic effects of NK cells against HepG2 cells were increased from (9.47±1.11)% and (20.45±1.94)% in the untreated groups to (28.88±1.23)% and (44.93±157)% in the sunitinib treatment groups (P<0.05). The expression of MICA, MICB and ULBP2 mRNA in HepG2 cells was also significantly elevated after treated with sunitinib. Conclusion: Sunitinib regulates the expressions of NKG2DLs (MICA/B and ULBP2) on HepG2 cells, which activates NK cells and is responsible for their enhanced cytotoxic action.
Keywords:
Project Supported:
Project supported by the National Natural Science Foundation for Young Scientists of China (No. 81302372，81300431), and the Excellent Middle and Yong Aged Experts Project of Zhujiang Hospital Southern Medical University（No. 2012007008)
Clc Number:
