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Archive > Volume 22 Issue 6 > 2015,22(6):729-733. DOI:10.3872/j.issn.1007-385X.2015.06.009 Prev Next
Basic Research
The apoptosis of non-small cell lung cancer cells induced by Artemisinin and its possible mechanism
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Abstract:
Objective:To investigate the effect of Artemisinin on apoptosis of non-small cell lung cancer (NSCLC) ASTC-a-1 and A549 cells and its mechanism. Methods:CCK-8 assay was performed to assess the effect of treatment with Artemisinin at 0-150 μg/ml for 24 h and at 100 μg/ml for 0, 6, 12, 24, 48 h on the activity of ASTC-a-1 and A549 cells. Laser scanning confocal microscope was used to observed influence of treatment with STS at 1 μg/ml, Artemisinin at 100 μg/ml alone or combined with NAC for 24 h on nuclear morphologies, and flow cytometry was used to examine impact of treatment with Artemisinin alone or combined with NAC on apoptosis of the cells; After silencing of Bax and Bak genes by RNA interference technology, effect of Artemisinin on viability of the cells was examined.Results: Artemisinin induced growth inhibition of ASTC-a-1 and A549 cells in a concentration and time dependent manner (IC50≈100 μg/ml), and the falling level of viability of the cells induced by Artemisinin was significantly less than untreaded cells after pretreated with NAC (all P<0.01). Treatments of ASTC-a-1 and A549 cells with STS, Artemisinin alone or combined with NAC induced their karyopyknosis and apoptosis. After pretreatment with NAC, the apoptosis rates of ASTC-a-1 and A549 cells induced by Artemisinin were (20.4±2.1)% and (17.9±3.8)% respectively, which were obviously less than STS group ( \[48.2±2.6\]% and \[39.8±4.9\]%, respectively) and without pretreated group (\[59.6±3.4\]% and \[50.7±3.8\]% respectively) (all P<0.01). Artemisinin only increased viability of silencing Bak cells (P<0.01), but not obviously impact that of silencing Bax cells (P＞0.05). Conclusion: Artemisinin induces apoptosis of non-small cell lung cancer ASTC-a-1 and A549 cells, which requires the participation of reactive oxygen species (ROS) and promoting apoptosis factor Bak, but not Bax.
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Project Supported:
Project supported by the Medical Science Research Project of Henan Province (No. 2011020091)
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