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Archive > Volume 22 Issue 6 > 2015,22(6):754-759. DOI:10.3872/j.issn.1007-385X.2015.06.013 Prev Next
Clinical Research
Expression and methylation status of lncRNA XLOC_008370 gene in esophageal squamous cell carcinoma and their relationship with clinical pathological features
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Abstract:
Objective:To investigate the expression and methylation status of lncRNA XLOC_008370 gene in esophageal squamous cell carcinoma（ESCC）and explore their role in ESCC development. Methods: RT-PCR and methylation specific PCR (MSP) were used to examine the expression and methylation status of XLOC_008370 gene in esophageal cancer cell lines (TE1, TE13, T.TN, Eca109, Yes-2) and in primary ESCC tissues. Their relationships with clinical pathological feature were further analyzed.Results: All 5 lines of esophageal cancer cells express no or low level of XLOC_008370, which was increased significantly after exposed to 5-Aza-dC, a DNA methyltransferase inhibitor. While the XLOC_008370 gene was highly methylated in all the 5 lines of cells, treatment with 5-Aza-dC led to decreased methylation of the gene in the Eca109 and Yes-2 cells, and completely demethylation in the TE1, TE13, and T.TN cells. In primary ESCC tumor tissues, the level of XLOC_008370 was significantly lower compared to that in corresponding non-cancerous tissues (P<0.05), and the expression was associated with TNM and pathological stages (P<0.05). The methylation frequency of XLOC_008370 gene promoter in ESCC tissues (54.02%, 47/87) was significantly higher than that in corresponding normal tissues (9.20%, 8/87) (P<0.05), and it was also associated with TNM and pathological stages (P<0.05). Furthermore, the level of XLOC_008370 in ESCC tumor tissues having its gene methylated was significantly lower than that in tumor tissues having the gene unmethylated (P<0.05). Conclusion: 〗Aberrant low expression of lncRNA XLOC_008370 is closely related to the development of ESCC, and promoter methylation is likely one of the mechanisms responsible for its decreased expression.
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Project Supported:
Project supported by the Science and Technology Plan Projects of Hebei Province (No. 122777117)，and the Medical Research Major Projects of Hebei Province (No. \[2012\]2056)
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