Mobile website
Basic Research
Notoginsenoside R1 enhances apoptosis of human leukemia cell line HL-60 through mitochondria-dependent pathway
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
Objective:To explore the effect of Notoginsenoside R1 on the apoptosis of human leukemia cell line HL-60 and possible mechanisms. Methods：After HL-60 cells were incubated with different concentrations of Notoginsenoside R1 (10, 20, 40 and 80 μmol/L) for 12, 24, 36 h and 48 h, apoptosis of the HL-60 cells was determined by MTT and Annexin V-FITC flow cytometry assays respectively. The expression of Bcl-2, Bax and cytochrome C (Cyt-C) in the HL-60 cells was determined by Western blotting. The change of mitochondrial membrane potential was assessed by JC-1 dyeing method. Results：The results of MTT and flow cytometry assays showed that Notoginsenoside R1 induced the apoptosis of HL-60 cells in dose-dependent manners, and survival rate of the HL-60 cells decreased with increase of incubation time; after adding Notoginsenoside R1, expression of Bcl-2 protein in the HL-60 cells remarkably decreased (\[0.45±0.03\] vs \[1.00±0.00\]，P<0.05, compared with control group); expression of Bax protein significantly increased (\[1.72±0.08\] vs \[1.00±0.00\]，P<0.05, compared with control group); and the ratio of Bcl-2/Bax decreased (\[0.21±0.01\] vs \[1.00±0.00\]，P<0.05); the mitochondrial membrane potential decreased (\[0.56±009\] vs \[1.00±0.00\]，P<0.05)；and expression level of Cyt-C protein in cytoplasm remarkably decreased (\[0.42±0.03\] vs \[1.00±0.00\]，P<0.05\]. Conclusion：Notoginsenoside R1 can remarkbly incluce apoptosis of HL-60 cells. Mitochondria-dependent pathway may be involved inNotoginsenoside R1-induced apoptosis of HL-60 cells. These findings suggest that Notoginsenoside R1 may be a new strategy for treatment of leukemia.
Keywords:
Project Supported:
Project supported by the Foundation from Heath and Family Planning Commission of Hebei Provine（No.20150238）
Clc Number:
