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Archive > Volume 23 Issue 2 > 2016,23(2):201-205. DOI:10.3872/j.issn.1007-385X.2016.02.008 Prev Next
Basic Research
Epigenetic mechanism of down-expression of miR-129-5p in human glioma tissues
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Abstract:
Objective:To explore the expression characteristics of miR-129-5p (matured miR-129) in human glioma tissues, and to investigate whether the abnormal expression of miR-129-5p in human glioma tissues is related to methylation of its promoter. Methods: Twenty one tumor tissue specimens and twenty one non-tumor tissue specimens were respectively collected from patients with glioma and patients with brain trauma, who underwent surgery in Department of Neurosurgery, Xiangya Hospital during October to December, 2013. Expressions of miR-129-5 in human glioma tissues, nontumor brain tissues, glioma cell lines and normal glial cell lines were measured with Real-time PCR.Promoter methylation of miR-129-2 gene in glioma tissues was examined by methylation-specific PCR (MSP). Changes of promoter methylation level of miR-129-2 gene in the glioma tissues after treatment of methylation transferase inhibitor 5-aza-2′-deoxycitydine (5-Aza-dC) were analyzed with bisulfite-sequencing PCR (BSP). Results: The expressions of miR-129-5p in human giloma tissues and cells were significantly lower than those in normal brain tissues and cells (P<0.05). The promoter methylation rates of miR-129-2 gene in glioma tissues were significantly higher than those in normal brain tissues (P<0.05). After treatment of demethylation with 5-Aza-dC, the promoter methylation of miR-129-2 gene decreased and the expression of miR-129-5p significantly increased (P<0.05). Conclusion: miR-129-5p is significantly down-expressed in human giloma, promoter methylation of miR-129-2 is one of the mechanisms which result in low expression of miR-129-2 in human glioma.
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Project Supported:
Project supported by the Research Foundation from the Bureau of Education of Hunan Province（No. 13C571）
Clc Number:
