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Archive > Volume 23 Issue 2 > 2016,23(2):218-222. DOI:10.3872/j.issn.1007-385X.2016.02.011 Prev Next
Basic Research
Effect of siRNA silencing CDX2 gene combined with chemotherapy drugs on the proliferation and apoptosis of leukemia K562 cells
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Abstract:
Objective:To investigate the effect of siRNA targeted silencing CDX2 gene combined with vincristine (VCR) on the proliferation and apoptosis of leukemia K562 cells. Methods: Specific siRNA sequence targeted silencing CDX2 gene (CDX2-siRNA-921) and its negative control sequence (CDX2-siRNA-NC) were designed and transfected into K562 Cells respectively. At the same time, untransfected K562 cells were used as a control group. The effect of CDX2-siRNA-921 transfection on the expressions of CDX2，BCR-ABL mRNA and CDX2 protein was detected by Real-time PCR and Western blotting respectively. After action of siRNA and VCR alone or in combination, proliferation inhibition rate and apoptosis rate of the K562 cells were respectively detected by MTT and flow cytometry assays. Results: Compared with the control group, CDX2-siRNA-921 significantly decreased the expressions of targeting gene CDX2 and BCR-ABL mRNA, and CDX2 protein (all P<0.05). But the expressions of CDX2 and BCR-ABL mRNA, and CDX2 protein in CDX2-siRNA-NC group had nonsignificant changes. Compared with CDX2-siRNA-NC、VCR and VCR+CDX2-siRNA-NC groups, the proliferation inhibition rate of the K562 cells significantly decreased and apoptosis rate of the cells significantly increased in VCR+CDX2-siRNA-921 group (all P<0.05). Conclusions: CDX2-siRNA-921 could obviously decrease the expressions of mRNA and protein of CDX2 gene and down-regulate the expression of BCR-ABL fusion gene in the K562 cells, as well as enhance the effects of VCR on proliferation inhibition and inducing apoptosis of K562 cells.
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Project Supported:
Projects supported by the Medical and Health Technology Development Program of Shandong Province（No.2014WS0183），and the Project of Natural Science Foundation of Shandong Province（No.ZR2014HL032）
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