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Archive > Volume 23 Issue 2 > 2016,23(2):223-229. DOI:10.3872/j.issn.1007-385X.2016.02.012 Prev Next
Basic Research
Roles of CREB and p-CREB in proliferation of prostate cancer cells
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Abstract:
Objective:To investigate roles of cAMP-response element binding protein (CREB) and phosphorglated CREB (p-CREB) in proliferation of prostate cancer cells.Methods:Tissue microarray technology was adopted to examine the expression levels of CREB and p-CREB in normal prostate tissue, hyperplasia prostate tissue and prostate cancer tissues at different grades. Immuno fluorescence staining and Western blotting were performed to detect the expression levels of CREB and p-CREB in human normal prostatic stromal immortalized WPMY-1 cell line, prostate cancer PC3 and LNCap cell lines. Recombinant human plasmid CREB was constructed and transfected into the PC3 and LNCap cells. Efficiency of the transfection and proliferation changes of the cells were verified by Western blotting and CCK-8 kit respectively. Results: Expression rates of CREB and p-CREB in prostate cancer tissue were significantly higher than those in normal prostate tissue (96% vs 50% and 88% vs 40%, P<0.05) and hyperplasia prostate tissue (96% vs 80% and 88% vs 60%, P<0.05). Expression levels of CREB and p-CREB proteins in the prostate cancer PC3 and LNCap cell lines were significantly higher than those in the normal prostatic stromal immortalized WPMY-1 cell line \[(5.10±0.62 vs 2.31±040), (7.5±0.83 vs 2.31±0.40), (4.31±0.54 vs 0.02±0.38), (6.15±0.69 vs 2.02±0.38), all P<0.05\]. Transfection with recombined CREB plasmid could up-regulate the expression of proliferating cell nuclear antigen (PCNA) in the prostate tumor PC3 and LNCap cells (all P<0.05), and proliferation levels of the PC3 and LNCap cells significantly increased after the transfection (P<0.05). Conclusion: The CREB and p-CREB were highly expressed in human prostate cancer tissues and, the prostate cancer PC3 and LNCap cells cultured in vitro. In addition, the CREB and p-CREB could up-regulate the expressions of proliferation-related gene PCNA, and raise proliferation level of the cells.
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Project Supported:
Project supporteded by the National Natural Science Foundation of China（No.81272619）
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