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Archive > Volume 23 Issue 2 > 2016,23(2):230-234. DOI:10.3872/j.issn.1007-385X.2016.02.013 Prev Next
Basic Research
inhibit the proliferation and migration of human gastric adenocarcinoma SGC-7901 cells through down-regulating the expression of mTOR
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Abstract:
Objective:To investigate the expression of has miR-99b in gastric adenocarcinoma cell lines and its potential mechanism of affecting proliferation and migration of the gastric adenocarcinoma cell lines. Methods: Real-time PCR was performed to detect the expressions of miR-99b in human gastric epithelial cell line GES-1 and human gastric adenocarcinoma cell line (SGC7901, BGC823 and MGC-803). The synthesized miR-99b mimics were transfected into the SGC-7901 cells to constract a overexpressed cell line, and scramble mimics were transfected into the same cells as control group. Then proliferation and migration of the SGC-7901 cell lines which overexpressed miR-99b were measured by CCK-8 assay and transwell assay; expression level of mTOR (mammalian target of rapamycin) protein was determined by Western blotting. Results: The expression levels of miR-99b in the gastric adenocarcinoma cell lines, especially in the SGC-7901 cell line, were significantly lower than those in the human gastric epithelial cell line. After transfectio of the miR-99b mimics for 48 h, proliferation ability of the SGC-7901 cell in experiment group was lower than that of control group（0.20±0.00 vs 0.27±0.02，P<0.05）. Compared to the cell line transfected with the scramble mimics, the experiment group showed a significant reduction in number of transmembrane cells（226.27±25.17 vs 523.33±25.17，P<005）after the transfection for 24 h. miR-99b can downregulate the expression of mTOR protein in the human gastric adenocarcinoma cell line SGC-7901. Conclusion:The expression of miR-99b in gastric adenocarcinoma cell lines was obviously down regulated, inhibition effect of miR-99b on proliferation and migration of the human gastric adenocarcinoma cell line SGC-7901 might be related with down-regulated expression of mTOR.
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Project Supported:
Project supported by the National Natural Science Foundation of China (No.81270465)，and the Program from the Bureau of Health of Chengdu (No.2013059)
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