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Archive > Volume 23 Issue 2 > 2016,23(2):255-260. DOI:10.3872/j.issn.1007-385X.2016.02.017 Prev Next
Clinical Research
Influence of combined action of anti-CD3 monoclonal antibody and anti-CD28 monoclonal antibody on proliferation and function of CIK cells and its subpopulations
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Abstract:
Objective:To explore the effect of combined action of anti-CD3 and anti-CD28 monoclonal antibodies on the proliferations and functions of cytokine-induced killer (CIK) cell and its subsets. Methods: Twenty peripheral blood samples of the patients with tumors (6 cases of renal carcinoma, 5 cases of lung cancer, every 2 cases of hepatic carcinoma, breast cancer and melanoma, and every 1 case of gallbladder carcinoma, lymphoma and ovarian cancer) who received biotherapy at the First Hospital Affiliated to Zhengzhou University from June 2014 to June 2015 were collected. Each sample of the peripheral blood was divided into four groups on average, namely control group, anti-CD3 monoclonal antibody alone group, anti-CD28 monoclonal antibody alone group and anti-CD3 combined with anti-CD28 monoclonal antibodies group, which were cultured with CIKs. Proliferation of the CIK cells was detected with CFSE staining. Secretion levels of IFN-γ, TNF-α and IL-2 by the CIK cells were detected by ELISA. CD4+, CD8+ and CD56+ cells of CIK cell subsets were sorted with magnetic beads. Then their abilities of secreting IFN-γ, TNF-α and IL-2 were examined by ELISA assay. Results:No significant difference in the proliferation ability of the CIK cells was detected between the anti-CD3/anti-CD28 combined group (PI=59.35) and anti-CD3 alone group (PI=64.4). However, combination of anti-CD3 and anti-CD28 monoclonal antibodies could stimulate CIK cells to secrete higher level of IFN-γ and TNF-α than those done by anti-CD3 alone, IFN-γ: (384.6±263.1) pg/ml vs (201.5±271.5)pg/ml, P=0.0361; TNF-α (4.795±1.251)pg/ml vs (2.835±0.443)pg/ml, P=0.0265. Furth analysis found that the combined action of anti-CD3 and anti-CD28 monoclonal antibodies could enhance activity of CD8+ cell subset to secret IFN-γ and activity of CD56+ cell subset to secret IL-2, thus strengthen the functions of lymphocytes. Conclusion: The combined action of anti-CD3 and anti-CD28 monoclonal antibodies could not enhance proliferation of the CIK cells, but strengthen function of the CIK cells to a certain extent, which has certain potential to be used in the immunotherapy of tumor cells.
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Project Supported:
Project supported by the National Natural Science Foundation of China (No.81271815,81171986), the National Natural Science Foundation of China and Sino-US Cooperation in Biomedical Research Projects (No.812111102), the Research Grant from the Ministry of Public Health (No.20110110001), and the Basic and Advanced Technology Research Foundation from Department of Science and Technology of Henan Province (No.112300410153,122300410155)
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