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Archive > Volume 23 Issue 2 > 2016,23(2):276-281. DOI:10.3872/j.issn.1007-385X.2016.02.020 Prev Next
Clinical Research
Expressions of IDO and BIN1 in esophageal squamous cancer tissues and draining lymph nodes and their clinical significances
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Abstract:
Objective:To investigate the expressions of indoleamine 2,3-dioxygenase (IDO) and brdging integrator 1 (BIN1) in tumor tissues and tumor draining lymph nodes (TDLNs) of the patients with esophageal squamous cell carcinoma (ESCC) and their clinical significances. Methods: Pathological confirmed tumor tissues, carcinoma adjacent tissues and TDLNs were collected from 71 patients with ESCC who underwent tumor resection in the Department of Thoracic Surgery, the Fourth Hospital of Hebei Medical University between Apr. 2013 and Jul. 2014. The carcinoma adjacent tissues were set up as the control group for tumor tissue group, while the non-metastatic lymph nodes set up as the control group for metastatic lymph node group. The expression levels of IDO and BIN1 were detected with Real-time PCR and immunohistochemistry assays, and correlation between the two expressions and their relations with clinical characteristics of the patients were analyzed. Results: Compared to the non-metastatic group, the expression level of IDO mRNA and positive rate of IDO protein were remarkably higher (0.47±0.14 vs 0.22±0.09, P<0.01; 90.91% vs 67.35%, P=0.042), while the expression level of BIN1 mRNA and positive rate of BIN1 protein remarkably lower (0.15±0.11 vs 0.35±015, P<0.01; 50.00% vs 77.55%, P=0.028) in metastatic group. In addition, the expression level of IDO mRNA and positive rate of IDO protein in carcinoma tissues were significantly higher than those in para-carcinoma tissues (0.51±0.12 vs 024±0.11, P<0.01; 81.69% vs 22.54%, P<0.01) while the expression level of BIN1 mRNA and positive rate of BIN1 protein significantly lower than those in para-carcinoma tissues (0.17±0.10 vs 0.41±0.14, P<001; 19.72% vs 80.28%, P=0.006). In both tumor tissues and TDLNs, the expression of IDO protein was associated with invasion range , lymph node metastasis and clinical stage of the tumors, while the expression of BIN1 protein was associated with degree of differentiation, invasion range, lymph node metastasis and clinical stage of the tumors. Conclusion: In ESCC tumor tissues and metastatic TDLNs, the expression level of IDO was significantly up-regulated and the expression level of BIN1 significantly down-regulated, which were closely associated with clinical features of the patients and might be important factors affecting progression of ESCC.
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Project Supported:
Project supported by the Young Scientists Foundation of the National Natural Science Foundation of China (No. H2012206135), and the Foundation for Distinguished Young Scholars of Hebei Province (No. H201406320)
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