Project supported by the National Natural Science Foundation of China(No. 81460358,81560380,81460441),the National High Technology Research and Development Program of China(863 Program)(No. 2011AA02A111) ,the Applied and Basic Research Foundation of Yunnan Provincial Science and Technology Department-Kunming Medical University(No. 2013FB165,2014FB066), the Yunnan Provincial Technology Project of Health(No.2014NS023,2014NS024),and the Zhan Qimin academician workstation of Yunnan Province