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Archive > Volume 23 Issue 3 > 2016,23(3):355-359. DOI:10.3872/j.issn.1007-385X.2016.03.010 Prev Next
Basic Research
Effects of advanced glycation end products on epithelial-mesenchymal transition and cancer stem cell associated markers in human colon cancer cell line SW620
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Abstract:
Objective:To investigate the effect of advanced glycation end products(AGEs) on epithelial-mesenchymal transition (EMT) and cancer stem cell associated marker CD133 in human colon cancer cell line SW620 and their mechanism of actions. Methods:After the SW620 cells were treated with AGEs at different concentrations of 0,50,100,200μg/ml, migration and invasion abilities of the SW620 cells were detected by wound healing test and Transwell chamber assay, respectively, percentage of CD133+ cells was tested by flow cytometry, and protein expression levels of receptor of AGEs(RAGE), E-cadherin, Vimentin, ERK1/2, p-ERK1/2 and CD133 were detected by Western blotting. Results: After the treatment of AGEs, compared with the control group (0 μg/ml), cellular migration distances in experiment groups (50, 100, 200 μg/ml) were significantly improved after 24 h (\[1.55±0.15\], \[1.58±0.19\], \[1.75±0.21\] vs \[0.95±0.18\] mm，all P<0.05) and 48 h （\[1.60±0.24\], \[2.11±0.22\], \[2.68±0.23\] vs \[1.60±0.24\] mm, all P<005). In addition, treating the cells with AGEs (50,100, 200 μg/ml) for 48 h remarkably increased number of the cells crossed Matrigel in vitro（ \[176±19.52\], \[194±17.70\], \[220±25.50\] vs \[125±26.06\]，all P＜0.05\] and percentages of CD133+ cell （\[4.75±1.49\], \[10.34±1.54\], \[14.45±2.41\]% vs \[0.77±0.41\]%，all P＜0.05). Expressions of RAGE, Vimentin, p-ERK1/2 and CD133 proteins were significantly increased in the treatment groups comparing with control group; however, the expression of E-cadherin protein was decreased, while that of ERK1/2 protein had not obvious change. Conclusion: AGEs could enhance migration and invasion abilities of the SW620 cells in vitro, promote occurrence of EMT, and induce tumorigenesis of cancer stem cells. Activating ligand of AGE-RAGE, up-regulating p-ERK1/2 prtein and regulating expression of the EMT protein might be a possible mechanism for tumorigenesis of cancer stem cells.
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Project Supported:
Project supported by the Natural Science Foundation of Changqing （No.cstc2012jjA0038)
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