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Archive > Volume 23 Issue 3 > 2016,23(3):371-377. DOI:10.3872/j.issn.1007-385X.2016.03.013 Prev Next
Clinical Research
The relationship of microsatellite instability with infiltration of T lymphocytes in tissues of non-small cell lung cancer
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Abstract:
Objective:To analyze expressions of the mismatch repair proteins, MLH1, MSH2 and MSH6, and infiltration of T lymphocytes in tissues of non-small cell lung cancer (NSCLC) and to explore a relationship of microsatellite instability (MSI) with infiltration of T lymphocytes in NSCLC. Methods:One hundred samples of NSCLC tissues diagnosed in Cancer Institute and Hospital, Tianjin Medical University between 2004 and 2010 were collected. Expressions of the MLH1, MSH2 and MSH6, and infiltration of T lymphocytes in the carcinoma tissues were examined with immunohistochemical assay. The carcinoma tissues with one negative expression among the above proteins were determined as MSI, and clinical pathologic characteristics of MSI NSCLC were analyzed. Results: Petection rate of MSI in NSCLC tissues was 24%, that was lower than that in MSS. Infiltration of T lymphocytes in the tissues of MSI NSCLC was obviously higher than that in MSS. Results of the immunohistochemical assays showed that numbers of CD3+, CD4+ and CD8+ T lymphocytes infiltrated in the tissues of MSI NSCLC were significantly higher than those in the tissues of MSS NSCLC (P<005). MSI situation in the cases was related with their age (P<0.05) but not with their gender, pathological type of tumor, primary tumor size, involvement of regional lymph node and distant metastasis (P>005). Conclusion: MSI affects the tumor immune microenvironment of NSCLC, which might provide a novel predictive indicator for immunotherapy of NSCLC.
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Project Supported:
Project supported by the National Basic Research Program of China(973 Program) (No.2012CB9333004), the National Natural Science Foundation of China (No.81272221), and the PhD Research Foundation from Cancer Hospital of Tianjin Medical University (No.B1309)
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