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Archive > Volume 23 Issue 3 > 2016,23(3):382-386. DOI:10.3872/j.issn.1007-385X.2016.03.015 Prev Next
Clinical Research
gene promoter methylation status and its clinical significance in esophageal squamous cell cancer tissues
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Abstract:
Objective:To detect the methylation status of Bin1 gene promoter and its mRNA expression in cancer tissues and para-carcinoma normal tissues from patients with esophageal squamous cell cancer (ESCC), and to explore its clinical significance. Methods: The expression level of Bin1 mRNA and its methylation status in ESCC tissues and the corresponding para-carcinoma normal tissues from 58 patients were determined by real-time quantitative RT-PCR (qRT-PCR) and methylation specific PCR (MSP). The relationship between Bin1 methylation status and clinical pathological staging was compared. Results: The methylation frequency of Bin1 in ESCC tissues was significantly higher than that in the corresponding para-carcinoma normal tissues (58.62% vs 25.86%, P<0.01), and the Bin1 methylation status was closely correlated with TNM stage, tumor invasion depth, tumor differentiation and lymph node metastasis (all P<0.05). Compared with para-carcinoma tissue specimens, the expression of Bin1mRNA in ESCC tissues was significantly lower (\[0.78±0.05\] vs \[1.03±0.03\], t=9.643, P<0.01) . The expression of Bin1 mRNA in ESCC tissues with methylation was significantly lower than that in ESCC tissues without methylation (\[0.68±0.04\] vs \[0.85±0.07\], t=2476, P＜0.05). Conclusion: Aberrant promoter methylation of Bin1 might be closely related to the development of ESCC, and it is one of the possible mechanisms that leads low expression or deletion of Bin1 in ESCC. The DNA methylation of Bin1 might be closely associated with tumor invasion and lymph node metastasis of ESCC.
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Project Supported:
Project supported by the National Natural Science Foundation of China (No.81201607), the Outstanding Youth Foundation of Hebei Province (No.H2014206320), and the Ｈigh Ｌevel Talents of Hebei Province (No.A201401040)
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