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Archive > Volume 23 Issue 5 > 2016,23(5):646-650. DOI:10.3872/j.issn.1007-385X.2016.05.010 Prev Next
Basic Research
Effect of shRNA interfering expression of CDX2 gene on hepatic metastasis of colorectal cancer in nude mice
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Abstract:
Objective:To establish a liver metastasis model of human colorectal cancer in nude mice，and to investigate the effect of interfering CDX2 gene on the metastasis of colorectal cancer in vivo. Methods： Lentiviral vector particles were used to infect human colorectal cancer HT29 and SW480 cells; CDX2 gene interfering cell lines (SW480-KD、HT29-KD) and negative shRNA were screened and established; cell lines with CDX2 gene interfering (SW480-KD,HT29-KD),cell lines with negative shRNA (SW480-NC, HT29-NC) and blank control cells (SW480-CON, HT29-CON) were injected into the spleen of nude mice, respectively; the ingestion, movement and mental status of the mice were observed everyday and the weight of nude mice were measured every days. The nude mice were sacrificed on D50 to observe the liver metastasis by visual and microscopic observation. Results：The liver metastasis model of human colorectal cancer in nude mice was successfully constructed and confirmed by H-E staining. The weight of rats in knockdown group (SW480-KD, HT29-KD) was significantly lower than that of the blank control group (SW480-CON, HT29-CON) and negative control group (SW480-NC, HT29-NC）(SW480：\[15.02±2.00\] g vs \[18.00±2.48\] g, \[18.03±2.05\] g, F=3.761, P<0.05; HT29: \[15.39±2.16\] g vs \[17.96±2.48\] g, \[18.30±1.87\] g, F=3.721, P<0.05). The number of liver metastatic tumors in SW480-KD group was significantly more than that of the SW480-CON group and SW480-NC group (\[5783±22.56\] vs \[29.50±16.90\], \[28.20±15.40\], F=4.197, P<0.05). The number of liver metastatic tumors in HT29-KD group was significantly more than that of the HT29-CON group and HT29-NC group (\[56.83±2916\] vs \[2640±12.76\] , \[21.00±11.50\]， F=4.467，P<0.05). Conclusion： The liver metastasis model in nude mice established by the spleen injection was an ideal model to study the geng function in vivo; the CDX2 gene interfering could promote the metastasis of CRC SW480 cells and HT29 cells in nude mice.
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Project Supported:
Project supported by the National Natural Science Foundation of China（No.81101874, No.81172362），the Science and Technology Project of Shaanxi Province (No.2016SF-015,No.2016SF-157,No.2015SF037），and the Co-ordinative and Innovative Plan Projects of the Science and Technology in Shaanxi Province (No.2013KTCQ03-08)
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