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Archive > Volume 23 Issue 5 > 2016,23(5):675-681. DOI:10.3872/j.issn.1007-385X.2016.05.015 Prev Next
Clinical Research
Expression and methylation status of lncRNA XLOC_002319 in esophageal squamous cell carcinoma
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Abstract:
Objective:To investigate the expression and methylation status of long non-coding RNA XLOC_002319 (lncRNA XLOC_002319) in esophageal squamous cell carcinoma (ESCC), and to elucidate its role in the progression of ESCC. Methods: Reverse transcription polymerase chain reaction (RT-PCR) and methylation specific PCR (MSP) were used respectively to detect the expression and methylation status of XLOC_002319 in esophageal cancer cell lines (TE1, TE13, Yes-2, Eca109, T.TN) treated or untreated with DNA methyltransferase inhibitor 5-aza-2′-detoxycytidine (5-aza-dC), and in cancer-adjacent normal tissues, esophageal intraepithelial neoplasia (EIN) tissues and ESCC tissues. Results:Negative or weak positive expression of XLOC_002319 was detected in the five esophageal cancer cell lines untreated with 5-aza-dC; however, after the treatment with 5-aza-dC, the expression of XLOC_002319 was enhanced. The methylation status of XLOC_002319 was highly expressed in esophageal cancer cell lines before the treatment of 5-aza-dc, however, after the treatment, the methylation level was decreased in Eca109 and T.TN cell lines, while the status in other three cell lines was negative. The expression of XLOC_002319 gene in ESCC tissues was significantly reduced compared to cancer-adjacent tissues and EIN tissues (P<0.01), and was closely associated with pathological differentiation and TNM stage (P<0.05). The methylation frequency of XLOC_002319promoter in ESCC tissues(63.75% \[51/80\]) was significantly higher than that in EIN tissues and adjacent normal tissues (P<0.01), and it was also associated with lymph node metastasis, pathological differentiation and TNM stage (P<0.05). The expressions of XLOC_002319 in ESCC tumor tissues with methylation of the gene was significantly lower than that in tumor tissues with unmethylation of the gene (P<0.01). Conclusion: Aberrant low expression of lncRNA XLOC_002319 was closely related to the development and occurrence of ESCC, and promoter methylation might be one of the mechanisms for inactivation of XLOC_002319 in ESCC.
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Project Supported:
Project supported by the National Natural Science Foundation of China（No.81572441）， and the Natural Science Foundation of Hebei Province（No. H2015206196）
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