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Archive > Volume 23 Issue 5 > 2016,23(5):688-691. DOI:10.3872/j.issn.1007-385X.2016.05.017 Prev Next
Clinical Research
Expression and clinical significance of PVT1 gene in blood of the patients with gastric cancer
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Abstract:
Objective:To examine expression level of human plasmacytoma variant translocation 1 (PVT1) gene and content of clinical commonly used tumor markers in blood of the patients with gastric cancer and to explore the relationship between PVT1 as well as tumor markers and clinical stages. Methods: Peripheral venous bloods of 51 cases with gastric cancer and 63 cases with chronic atrophic gastritis who hospitalized in the First Hospital Affiliated to School of Medicine, Shihezi University during January to December, 2015 as well as 54 normal persons were collected, from which total blood RNA was extracted. Real-time quatitative PCR (qRT-PCR) was used to detect expression levels of PVT1 mRNA in the bloods; contents of AFP, CEA and CA19-9 in the bloods of the patients with gastric cancer were examined by electrochemical luminescence assay. Results: Expression amounts of PVT1 mRNA in bloods of the patients with gastric cancer and chronic atrophic gastritis were higher, there was statistical difference of PVT1 mRNA expression amounts between gastric cancer group and normal group (t=0.000,P<0.01), there also was statistical difference of PVT1mRNA expression amounts between chronic atrophic gastritis group and normal group (t=0.000, P<0.01), but there was no statistic difference of PVT1 mRNA expression amounts between gastric cancer group and chronic atrophic gastritis group (t=0459, P>0.05). In addition, expression of PVT1 mRNAand serum tumor marker CA19-9 had correlation (r=0.429, P<0.01). Conclusion: Expression of PVT1 mRNA in blood of the patients with gastric cancer and chronic atrophic gastritis could be higher than that in blood of normal persons, and which might be correlated with CA19-9, indicating that PVT1mRNA might be as one of molecular markers for early diagnosis and prognosis.
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Project Supported:
Project supported by the Basic Research for Application of Xinjiang Province（No.2015AG015）, and the Research Project of High Level Talents of Shihezi University （No. RCZX201332）
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