Mobile website
Archive > Volume 23 Issue 6 > 2016,23(6):783-788. DOI:10.3872/j.issn.1007-385X.2016.06.008 Prev Next
Basic Research
Effect of monoclonal antibody to basic fibroblast growth factor combined with lobaplatin on proliferation and apoptosis of lung adenocarcinoma A549 cell
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
Objective:To explore effect of monoclonal antibody to basic fibroblast growth factor (bFGF mAb) combined with lobaplatin (LBP) on proliferation and apoptosis of lung adenocarcinama A549 cell and their possible mechanisms. Methods: Effect of the drugs on viability of the A549 cell was detected by CCK8 assay. Annexin V-FITC/PI assay was used to detect apoptosis rates of the A549 cell. Laser scanning confocal microscope was used to observe nuclear morphology of the cells. Expressions of related-apoptosis proteins in the cells were measured by Western blotting assay. Results: Outcomes of CCK8 assay shown that after culturing of the cell for 48 h, proliferations of the cells in bFGF mAb and LBP groups were inhibited, but inhibited proliferation of the cell in combination of both group was obviously higher than those in single LBP and single bFGF mAb groups (P<0.05). Results of Annexin V-FITC/PI assay suggested that early and later apoptosis rate in bFGFmAb combined with LBP group was (27.83±1.23)% and (39.32±1.01)% respectively, comparing with those in bFGF mAb group (\[6.25±0.19\]% and \[14.54±0.25\]%) and those in LBP group (\[1654±0.39\]% and \[21.01±0.98\]%), early and later apoptosis rates of the combination group was significantly higher than those of the single drug groups (P<0.01). The result of laser scanning confocal microscope showed that cytonuclear fragmentation rate of the combination drugs group was more significantly increased than those of the single LBP and the single bFGF mAb groups. Western blotting assay displayed that in the combination group, expressions of BAX, Caspase-3, Caspase-9 and PARPs proteins obviously increased and expressions of Bcl-2, cleaved-Caspase-3, cleaved-Caspase-9 and cleaved-PARP proteins obviously decreased as comparing with those in both of the single drug groups. Conclusion: bFGF mAb combined with LBP could inhibit proliferation of lung adenocarcinama A549 cell and induce apoptosis of the A549 cell via inhibiting expressions of Bcl-2, cleaved-Caspase-3, cleaved-Caspase-9 and cleaved-PARP proteins and up-regulating expression of BAX, Caspase-3, Caspase-9 and PAR proteins.
Keywords:
Project Supported:
Project supported by the National Natural Science Foundation of China (No. 81273814), and the Specific Project Foundation of Key Science and Technology for Development of New Medicine of Guangdong Province (No. 2013A022100031)
Clc Number:
