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Archive > Volume 23 Issue 6 > 2016,23(6):789-794. DOI:10.3872/j.issn.1007-385X.2016.06.009 Prev Next
Basic Research
Using human CIK cells labeled with gold nanorods for targeted photoacoustic imaging and immunotherapy of gastric cancer
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Abstract:
Objective:Using property of human cytokine-induced killer cells (CIK) targeting gastric cancer cells to achievie local photoacoustic imaging of gastric cancer, to observe distribution of gold nanorods in gastric cancer tissues and to measure concentration changes of cytokines in serum. Methods: Peripheral blood monocuclear cells were extracted from whole blood of healthy volunteers and CIK cells were amplified induced under inductions of multiple cytokines. A seed synthesis method was used to prepare gold nanorods and to modify their surfaces with silicon dioxide(20 nm thick). A nude mouse model with subcutaneous xenograft of gastric tumor was etablished using gastric cancer MGC803 line cells. The CIK cells labeled with gold nanorods were injected into caudal vein of the nude mice with xenograft of gastric cancer and distribution of the CIK cells labeled with gold nanorods in the tumor tissues was observed with photoacoustic imaging at various time points of the injection. After 3 days of the injection, concentrations of IL-2, IL-7 and γ-IFN in serum of the nude mice were detected by ELISA assay. Results:CIK cells and gold nanorods were successfully made. The gold nanorods with 60 nm long and 8 nm wide were swallowed by the human CIK. The human CIK cells labeled with the gold nanorods actively targeted gastric cancer tissues, a large number of them accumulated in the gastric cancer tissues and enhanced signaling of photoacoustic imaging was presented. Concentrations of cytokines IL-2, IL-7 and γ-IFN significantly increased, and were higher than those in the control group (P<0.05). Conclusion: The CIK cells labeled with the gold nanorods could actively target gastric cancer tissues, efficiently collect and present enhanced signaling of photoacoustic imaging. At the same time, cytokine level in blood could be elevated and curative efficacy of the immunotherapy could be enhanced.
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Project Supported:
Project supported by the National 973 Project Foundation (No. 2015CB931802），the National Natural Science Foundation of China ( No.81225010, 81028009），and the Shanghai Municipal Science Committee Foundation (No.13NM1401500)
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