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Archive > Volume 23 Issue 6 > 2016,23(6):795-800. DOI:10.3872/j.issn.1007-385X.2016.06.010 Prev Next
Clinical Research
Expressions of Efp and Plk3 in estroen receptor positive breast cancer and their clinical significance
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Abstract:
Objective:To explore the expressions of Efp and PlK3 in patients with estroen receptor (ER) positive breast cancer and to analyze their clinical significance and possible mechanisms of the actions. Methods: Immuno histochemistry was used to detect expressions of Efp and PlK3 proteins in ER positive breast cancer tissues of 86 cases who were hospitalized in Department of Breast Surgery, the 4th Hospital of Hebei Medical University during January to June, 2010. Correlation between expressions of the both protein and their relationship with clinical pathological features were analyzed. Expressions of ER and Efp mRNAs in breast cancer MCF-7 and MDA-MB-231 line cells were detected by qRT-PCR assay. RT-PCR and Western blotting assays were used to check expression changes of Efp and Plk3 genes in ER positive breast cancer MCF-7 line cell after stimulation of estrogen. Expression changes of Efp and Plk3 proteins in the MCF-7 cell after treatments with estrogen and protease inhibitor MG132 were detected by Western blotting assay. Results:In ER positive breast cancer tissues of the 86 cases, Efp expression of 55 cases was positive (64.0%) and PIk3 expression of 28 cases was positive (32.6%). Positive expression of PIk3 was 23.6% in the tissues of Efp positive expression and that was 48.4% in the tissues of Efp negative expression. There was a significant negative correlation between expressions of Efp protein and Plk3 protein (P<0.05). Expression of Efp protein and lymph node metastasis of the patients with breast cancer were obviously positive correlation (P<0.05), and expression of Plk3 protein and lymph node metastasis of the patients with breast cancer were obviously negative correlation （P<0.05). Expression of ER mRNA in the MCF-7 cell was high, but expression of ER mRNA in the MDA-MB-231 cell was low. After stimulated by estrogen, Efp mRNA expression of the MDA-MB-231 cell did not obviously changed. After stimulated by estrogen, mRNA and protein expressions of Efp gene of the MCF-7 cell significantly increased (P<0.05), however expressions of Plk3 mRNA and its protein did not markedly changed, expression of Plk3 protein was not detected also. After treated by MG132, expression of Plk3 protein in the MCF-7 cell evidently increased. After stimulated by estrogen, treatment of MG132 significantly increased expression of Efp protein and decreased expression of Plk3 protein in the MCF-7 cell (all P<0.05). Conclusion: There was obvious negative correlation between expressions of Efp and Plk3 proteins in the ER positive breast cancer. Efp protein could improve degradation of Plk3 protein, and could involve in drug resistance process of endocrine therapy for patients with ER positive breast cancer.
Keywords:
Project Supported:
Supported by the National Natural Science Foundation of China (No.81001178), and the Self-funded Project of Science and Technology Research and Development Plan of Hebei Province (No.152777184)
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