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Archive > Volume 23 Issue 6 > 2016,23(6):801-805. DOI:10.3872/j.issn.1007-385X.2016.06.011 Prev Next
Clinical Research
Analysis of N-linked glycans in breast carcinoma
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Abstract:
Objective:To analyze structures of specific glycans in breast carcinoma, in order to provide potential early diagnostic markers for breast carcinoma.Methods: Fifteen specimens of breast carcinoma and five specimens of tissues adjacent to the carcinoma margin 3-5 cm surgical resected from the patients with breast cancer who were hospitalized in Department of Galactophore, Tumor Hospital of Shaanxi Province during May 2014 to September 2014 were collected. In addition, four specimens of breast lobular hyperplasia tissues were selected. Tissue proteins were extracted. Using filter membrane assisted method for the release of glycoprotein glycans, N-linked glycans were obtained. Type distributions and content changes of glycoprotein N-linked glycans in breast carcinoma, adjacent breast carcinoma and normal breast tissues were analyzed by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF/TOF-MS) technique. Results: hirtyeight,thirty four and twentytwo N-linked glycans were respectively identified in the breast cancer tissues, the adjacent tissues, and the normal breast tissues. Comparing with the paracarcinoma tissues and the normal breast tissues, contents of complex, high mannose, bisecting, fucosylated and two attenna types of N-linked glycans in the breast carcinoma tissues significantly decreased except for that content of high mannose type N-linked glycans obviously increased (P<0.05 or P<0.01). In the breast carcinoma tissue, contents of two types of high mannose type N-linked glycans, relative molecular masses of that were 1 580.455 and 1 742.490 respectively, were significantly higher than those in the normal breast tissues (P<0.05 or P<0.01), and contents of two types of complex type N-linked glycans, relative molecular masses of that were 1 647.473 and 1 850.583 respectively, were obviously lower than those in the normal breast tissues (P<0.05 or P<0.01). ROC curve analysis showed that the 4 kinds of N-linked glycanshave high detection accuracy (P<0.05 or P<0.01). Conclusion: Four types of specific N-linked glycans found in the breast carcinoma could be expected to become potential markers for early diagnosis and target therapy of the breast carcinoma.
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Project Supported:
Project supported by the National Science Foundation for Young Scientists of China（No. 81402115）
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