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Archive > Volume 23 Issue 6 > 2016,23(6):806-812. DOI:10.3872/j.issn.1007-385X.2016.06.012 Prev Next
Clinical Research
Effect of abnormal expression of STRAP on differentiation of gastric cancer cells
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Abstract:
Objective:To explore effect abnormal expression of serine-threonine kinase receptor-associated protein (STRAP) on differentiation of gastriic cancer cells. Methods: Two hundred fifty three cases of surgically resected gastric carcinoma specimens in Department of Digestive Tumor Surgery, Tumor Hospital of Peking University during January 1991 to January 2009 were collected. At the same time, 103 cases of carcinoma adjacent tissue from the same lot of the patients with gastric carcinoma were collected as control. Tissue microarrays were prepared. Immuno histochemistry assay was used to detect expressions of STRAP protein in the gastric carcinoma tissues and the carcinoma adjacent tissues, and relationship between expressions of the STRAP protein and clinical pathological factors, namely differentiation of gastric carcinoma cells, clinical staging and so on was analyzed. Relationship between abnormal expression of STRAP protein and prognosis of the patients with gastric carcinoma was analyzed by Kaplan-Meier survival analysis. STRAP eukaryotic expression vectors were transfected into gastric carcinoma SGC7901 line cells. After the transfection, expressions of STRAP protein in the SGC7901 cells were examined by Western blotting, proliferation abilities of the SGC7901 cells were detected by MTT test, expressions of the gastric cancer cell differentiation associated-protein pepsinogen C (PGC) in the SGC7901 cells were checked by immuno fluorescence staining assay. Meanwhile activity of alkaline phosphatases (AKP）in the cells with excessive expression of STRAP was analyzed. Results: Positive expression rate of STRAP protein in the gastric carcinoma tissues was obviously lower than that in the carcinoma adjacent tissues (33.6% \[75/223\] vs 59.3% \[51/86\], P<001), and the expression level of STRAP was closely related to defferentiation grade of the gastric carcinoma cells (P<0.05), prognosis survival of the patients with high expression of STRAP protein was evidently longer than that of the patients with low expression of the protein (P<0.05). After overexpression of STRAP protein in the gastirc carcinoma SGC7901 cells, proliferation ability of the cells significantly attenuated (P<0.05), expression of the PGC obviously increased (P<0.05), and activity of the AKP evidently decreased (P<0.05). Conclusion: There could be a strong link between expression level of STRAP protein in the gastric carcinoma tissues and differentiation of the gastric carcinoma cells, the STRAP protein as a molecualr marker might have a good clinical value for differentiation grade of the gastric carcinoma cells and supplementary judgment of prognosis in the patients with gastric carcinoma.
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Project Supported:
Project supported by the Applied Basic Research Program of Xinjiang Production and Construction Corps（No.2015AG015）
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