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Archive > Volume 23 Issue 6 > 2016,23(6):824-829. DOI:10.3872/j.issn.1007-385X.2016.06.015 Prev Next
Clinical Research
Expression of galectin-9 esophageal squamous cell carcinoma and its relationship with clinical pathological features
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Abstract:
Objective:To explore expressions of galectin-9 in esophageal squamous cell cancer (ESCC) and para-carcinoma tissues and its effect on migration and invasion abilities of ESCC cells. Methods: Eighty nine samples of ESCC tissues and twenty samples of para-carcinoma tissues that are far from the the carcinoma tissue margin 5 cm or more which were resected from the patients with ESCC in Department of Thoracic Surgery, Ji’nan Central Hospital affiliated to Shandong University during January, 2012 to January, 2013 were collected. Immunohistochemistry assay was used to detect expressions of galectin-9 protein in the ESCC and the para-carcinoma tissues. Their relationships with clinical pathological features of the patients, such as genders, ages, invasion depths, tumor sizes, differentiated degrees, pathological stages (pTNM), lymphatic metastasis and so on were analyzed. pcDNA3.1-galectin-9 was transfected into human esophageal carcinoma EC9706 line cells with liposome mediated method. Effect of up-regulation expression of galectin-9 on migration and invasion abilities of the EC9706 cells were detected by cell scratch healing test and Transwell invasion assay. Results: Positive rate of galectin-9 protein expression in the ESCC tissues was obviously lower than that in the para-carcinoma tissues (25.8% \[23/89\] vs 50% \[10/20\], P<0.05). Expressions of galectin-9 protein and carcinoma differentiated degrees, lymphatic metastasis in the patients with ESCC related (all P<0.05), and genders, ages, tumor sizes, invasion depths, pTMN in the patients with ESCC did not related (P>0.05). After over expression of galectin-9 protein in the EC9706 cells, invasion and migration abilities of the carcinoma cells decreased significantly, comparing with negative control group (invasion ability: \[45.0±8.0\] vs \[160.0±12.0\], P<0.01; migration ability: \[20.6±2.1\] vs \[87.6±4.9\], P<0.05). Conclusion:Absence of galectin-9 expression might involve in occurrence and development of the esophageal carcinoma, galectin-9 could inhibit invasion and migration of the ESCC cells.
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