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Archive > Volume 24 Issue 2 > 2017,24(2):112-116. DOI:10.3872/j.issn.1007-385X.2017.02.002 Prev Next
Prompt Report
Biological activity and plasma stability of metastatic colorectal cancer LoVo cell specific aptamer W14
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Abstract:
Objective:To analyze the biological activity and plasma stability of specific aptamar W14 to metastatic colorectal cancer LoVo cell. Methods: Fluorescence microscopy was used to observe the specific binding activity of W14 to target LoVo cells under 25 ℃ and 37 ℃; Flow cytometry was used to detect W14 recognition ability of LoVo cell in plasma environment. W14 was respectively incubated in complete culture medium and human plasma for 0.5, 1, 1.5, 2, 3, 4 and 6 h under 37 ℃, and Agarose gel electrophoresis was used to observe the biological stability of aptamer W14; LoVo cells were treated with W14 at different concentrations (2，4，8，10 μmol/L) for 24, 48 and 72 h, and MTS was used to investigate the cytotoxicity of aptamer W14. Results: The aptamer W14 could specifically recognize the target LoVo cells under different temperatures and plasma environments, and it could stably exist in plasma for 6 hours without degradation, in addition, it had no obvious toxic effect on cells. Conclusion: Metastatic colorectal cancer-specific aptamer W14 is suitable for in vivo use, with superior adaptability, good biological stability and low toxicity.
Keywords:
Project Supported:
Project supported by the National Natural Science Foundation of China (No.81502703)
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